Adhesion of Streptococcus mutans to salivary proteins in caries-free and caries-susceptible individuals.
نویسندگان
چکیده
Adhesion of microorganisms to dental surfaces is the initial step in the formation of dental bacterial plaque. Streptococcus mutans (S. mutans) is considered the main causal agent of one of the most common diseases in humans: dental caries. Adherence of these bacteria results from the interaction of adhesins that form part of their structure with salivary components, specifically those that compose the acquired pellicle. The complexity of this interaction has been the subject of studies in past years, to the extent of identifying certain salivary components related to adhesion to enamel surfaces, such as proline-rich proteins (PRSs), Staherins, Histatins, Cystatins, etc. One of the objectives of this study was to determine the adhesion capacity of S. mutans to synthetic hydroxyapatite incubated with saliva samples of caries-active and caries-inactive individuals. For the purpose of these assays, both the whole saliva samples and the salivary protein extracts were used. They were obtained by separating the proteins contained in the simple SDS-PAGE, in three ranges of molecular weight, selected in accordance with the electrophoresis profile that was usually found. The results indicated that the adhesion of this microorganism was greater in caries-inactive patients, when tested with whole saliva and proteins in the 120-159 kDa molecular weight range. This suggests that adhesion, per se, does not have a definite effect on the mechanisms that cause the disease in some individuals. However, these are interesting findings that may contribute to the design of strategies to control the adhesion of S. mutans to the tooth's surface.
منابع مشابه
The Comparison of Proteins Elaborated by Streptococcus mutans Strains Isolated from Caries Free and Susceptible Subjects
متن کامل
The Frequency of Streptococcus Mutans and Lactobacillus spp.in 3-5-year- old Children with and without Dental Caries
Abstract Background and Objective: The high occurrence of early childhood caries (ECC) is one of the most common problems in children dentistry. Streptococcus mutans and lactobacilli.spp are closely associated with the development of early childhood caries (ECC). Therefore, the aim of this study was to investigate the frequency of streptococcus mutans and lactobacilli.spp in 3-5 –year- old ch...
متن کاملSalivary immunoglobulin A and serum antibodies to Streptococcus mutans ribosomal preparations in dental caries-free and caries-susceptible human subjects.
Caries-free subjects or individuals with low caries susceptibility exhibited significantly higher (P less than 0.001) levels of naturally occurring salivary immunoglobulin A (IgA) and serum IgG, IgA, and IgM antibodies to a Streptococcus mutans ribosomal preparation than subjects with high caries susceptibility. Absorption of saliva and serum samples with S. mutans ribosomal preparations, but n...
متن کاملAntigenic characterization of fimbria preparations from Streptococcus mutans isolates from caries-free and caries-susceptible subjects.
The adhesion of pathogenic bacteria to the host surface is an essential step in the development of numerous infections, including dental caries. Attachment of Streptococcus mutans, the main etiological agent of human dental caries, to the tooth surface may be mediated by glucan synthesized by glucosyltransferase (GTF) and by cell surface proteins, such as P1, which bind to salivary receptors. F...
متن کاملSalivary Streptococcus mutans and Lactobacilli Levels as Indicators of Dental Caries Development in Iranian Patients with Systemic Sclerosis
Background and Objective: Systemic sclerosis is an autoimmune disorder with orofacial manifestations, including tooth decay. Lactobacilli can inhibit biofilm formation and growth of cariogenic pathogens, such as Streptococcus mutans. We aimed to assess the salivary levels of S. mutans and Lactobacilli as indicators of dental caries development in patients with systemic sclerosis. Methods: In t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Acta odontologica latinoamericana : AOL
دوره 19 2 شماره
صفحات -
تاریخ انتشار 2006